Why the AMES test does not say DNP is safe

[Ceebs]

ANd I'm not positive the demand is high enough...

Chalk another line on the high demand tally from me. This information would be invaluable to anyone who's ever used DNP, or thought about it. Even if I didn't have a personal reason for wanting to know, I'd still think it's interesting from a purely scientific point of view.

 

[Andy13]

Andy,
Would a change in p53 be the only other indicator of a cell line possibly becoming malignant? Im assuming that there are no other mutations caused by DNP, so it would have to be a change to a tumor suppressor gene...or some other important gene?
PS.... wouldnt that gene need to be downregulated or turned off if it is a tumor supressor gene?

All the abstracts i have been reading have been DNPs effects in more of a broad sense, such as its effects on various intra and extra cellular reactions, not on the DNA level, so i agree, this test of yours would be quite informative.

It often takes time (days, months, years?) of exposure to some carcinogen before it acutally causes malignant growth.. A Western blot for p53 is a quick and very good way to take a snapshot of the cell and get an idea of what's going on. p53 is responsible for causing DNA-repair enzymes to be up-regulated, it also induces proteins needed for cycle arrest and apoptosis.. P53 is truely the biological police detective. Just like a bunch of cop cars showing up in your neighborhood is usually a good indicator that something is not quite right, an increase in p53 from the low-low levels normally seen in a cell is likewise a signal that the cell is under some sort of genomic threat, uv light, carcinogens, etc. Actually, DNA strand breaks themselves are known to induce p53. Detection of this 'first response' against cell damage by measuring p53 induction is used quite often in oncology.

Andy

 

[bigrand]

So, this gene, if overactive, is a signal that there is some sort of potential damage to the DNA? This test will tell us that DNP is/isnt causing some sort of genomic stress, correct?

Damn, i thought we would have to find WHERE the damage took place and if it was a missense, nonsense, frameshift mutation, whatever.....This is the OVERALL indicator of genomic damage, i guess specifics arent neccesary, only when determining how to treat malignant tissue..?

 

[Andy13]

So, this gene, if overactive, is a signal that there is some sort of potential damage to the DNA? This test will tell us that DNP is/isnt causing some sort of genomic stress, correct?

Damn, i thought we would have to find WHERE the damage took place and if it was a missense, nonsense, frameshift mutation, whatever.....This is the OVERALL indicator of genomic damage, i guess specifics arent neccesary, only when determining how to treat malignant tissue..?

There's a chance that DNP could induce p53 while no genomic damage is taking place, likewise, there is also a chance that DNP could be a major threat to the cell while p53 is NOT induced.. But these possibilities are rather small. Induction of p53 classically occurs when the cell is under attack. Without this gene, DNA repair is compromised with a reduced ability to arrest growth and/or commence cell suicide (since p53 plays well-known roles in these processes).

 

[bigrand]

Curious, if p53 is active it can repair DNA if damaged and "induce apoptosis", correct?
Why when in malignant cell line with overexpressed p53, there is no apoptosis? Is it because the p53 gene is mutated to where there is no apoptosis, but the genen is still active in DNA repair?

Thinkin about this one for a few days ive been....

 

[Andy13]

Curious, if p53 is active it can repair DNA if damaged and "induce apoptosis", correct?
Why when in malignant cell line with overexpressed p53, there is no apoptosis? Is it because the p53 gene is mutated to where there is no apoptosis, but the genen is still active in DNA repair?

Thinkin about this one for a few days ive been....

It could be the case that one of the downstream mediators of apoptosis is non-functional.. Also, I'm sure there are a host of cellular triggers that influence p53's decision to induce apoptosis, or cell cycle arrest. Additionally, post-transcriptional regulation of p53 is very important, and helps the protein to become active and also regulates it's half life..

Andy

 

[bigrand]

Well, one thing is for sure, its still hands down the best fat burner out there, nothing gives me the results this shit does.
That is unless someone can get me some Compound C75......!!!!
Damn Andy, you have me wanting to be an Oncologist again!

 

[OXANDRIN]

bump

 

[bigrand]

Well Andy, with whats known now about DNP and its dangers, would you say that it can still be used relitivly safely? I ask becasue nothing comes close to DNP in terms of fat loss effectiveness, nothing, and ive tried a lot......

 

[bigrand]

AAAAAAnnnnnndddrrreeeewwww...........