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Thymosin Beta 4(TB-500) Injury Healing
- Thread starter John Juan
- Start date
John Juan
New member
TB500 is a synthetic version of Thymosin Beta-4 which does all the same wonderful things that its natural counterpart does. Some of the claims made by makers of TB500 include:
*Endothelial (blood vessels) cell differentiation
*Angiogenesis (growth of new blood cells from pre-existing vessels) in dermal tissues
*Keratinocyte migration
*Collagen deposition; and
*Decreases inflammation.
*Endothelial (blood vessels) cell differentiation
*Angiogenesis (growth of new blood cells from pre-existing vessels) in dermal tissues
*Keratinocyte migration
*Collagen deposition; and
*Decreases inflammation.
John Juan
New member
A novel dimeric thymosin beta 4 with enhanced activities accelerates the rate of wound healing.
AuthorsXu TJ, et al. Show all Journal
Drug Des Devel Ther. 2013 Oct 1;7:1075-88. doi: 10.2147/DDDT.S50183.
Affiliation
Abstract
OBJECTIVE: Thymosin beta 4 (Tβ4) is a peptide with 43 amino acids that is critical for repair and remodeling tissues on the skin, eye, heart, and neural system following injury. To fully realize its utility as a treatment for disease caused by injury, the authors constructed a cost-effective novel Tβ4 dimer and demonstrated that it was better able to accelerate tissue repair than native Tβ4.
METHODS: A prokaryotic vector harboring two complete Tβ4 genes with a short linker was constructed and expressed in Escherichia coli. A pilot-scale fermentation (10 L) was performed to produce engineered bacteria and the Tβ4 dimer was purified by one-step hydrophobic interaction chromatography. The activities of the Tβ4 dimer to promote endothelial cell proliferation, migration, and sprouting were assessed by tetramethylbenzidine (methylthiazol tetrazolium), trans-well, scratch, and tube formation assays. The ability to accelerate dermal healing was assessed on rats.
RESULTS: After fermentation, the Tβ4 dimer accounted for about 30% of all the bacteria proteins. The purity of the Tβ4 dimer reached 98% after hydrophobic interaction chromatography purification. An average of 562.4 mg/L Tβ4 dimer was acquired using a 10 L fermenter. In each assay, the dimeric Tβ4 exhibited enhanced activities compared with native Tβ4. Notably, the ability of the dimeric Tβ4 to promote cell migration was almost two times higher than that of Tβ4. The rate of dermal healing in the dimeric Tβ4-treated rats was approximately 1 day faster than with native Tβ4-treated rats.
CONCLUSION: The dimeric Tβ4 exhibited enhanced activity on wound healing than native Tβ4, and the purification process was simple and cost-effective. This data could be of significant benefit for the high pain and morbidity associated with chronic wounds disease. A better strategy to develop Tβ4 as a treatment for other diseases caused by injuries such as heart attack, neurotrophic keratitis, and multiple sclerosis was also described.
AuthorsXu TJ, et al. Show all Journal
Drug Des Devel Ther. 2013 Oct 1;7:1075-88. doi: 10.2147/DDDT.S50183.
Affiliation
Abstract
OBJECTIVE: Thymosin beta 4 (Tβ4) is a peptide with 43 amino acids that is critical for repair and remodeling tissues on the skin, eye, heart, and neural system following injury. To fully realize its utility as a treatment for disease caused by injury, the authors constructed a cost-effective novel Tβ4 dimer and demonstrated that it was better able to accelerate tissue repair than native Tβ4.
METHODS: A prokaryotic vector harboring two complete Tβ4 genes with a short linker was constructed and expressed in Escherichia coli. A pilot-scale fermentation (10 L) was performed to produce engineered bacteria and the Tβ4 dimer was purified by one-step hydrophobic interaction chromatography. The activities of the Tβ4 dimer to promote endothelial cell proliferation, migration, and sprouting were assessed by tetramethylbenzidine (methylthiazol tetrazolium), trans-well, scratch, and tube formation assays. The ability to accelerate dermal healing was assessed on rats.
RESULTS: After fermentation, the Tβ4 dimer accounted for about 30% of all the bacteria proteins. The purity of the Tβ4 dimer reached 98% after hydrophobic interaction chromatography purification. An average of 562.4 mg/L Tβ4 dimer was acquired using a 10 L fermenter. In each assay, the dimeric Tβ4 exhibited enhanced activities compared with native Tβ4. Notably, the ability of the dimeric Tβ4 to promote cell migration was almost two times higher than that of Tβ4. The rate of dermal healing in the dimeric Tβ4-treated rats was approximately 1 day faster than with native Tβ4-treated rats.
CONCLUSION: The dimeric Tβ4 exhibited enhanced activity on wound healing than native Tβ4, and the purification process was simple and cost-effective. This data could be of significant benefit for the high pain and morbidity associated with chronic wounds disease. A better strategy to develop Tβ4 as a treatment for other diseases caused by injuries such as heart attack, neurotrophic keratitis, and multiple sclerosis was also described.
John Juan
New member
Thymosin Beta 4, also known as TB500 or TB-500, is a synthetic version of the naturally occurring peptide present in virtually all-human and animal cells, Thymosin Beta 4 (T4). It is a first-in-class candidate that promotes the following*:
WHAT THIS DOES:
1. Increases Red Blood Cells
2. Stops bleeding
3. Increase Endurance
4. Reduces Tie Up
5. Helps breathing
6. Reduces stomach acid which eliminates ulcers
7. Increases lean muscle mass
8. Helps repair tendons and ligaments
WHAT THIS DOES:
1. Increases Red Blood Cells
2. Stops bleeding
3. Increase Endurance
4. Reduces Tie Up
5. Helps breathing
6. Reduces stomach acid which eliminates ulcers
7. Increases lean muscle mass
8. Helps repair tendons and ligaments
John Juan
New member
TB-500 is huge in the world of horse racing. Many a race was fixed by a TB-500 doped horse. My belief is there is huge money in horse racing so you know this is a good peptide. They aren't going to risk giving a million dollar horse some crap supplement when so much is at stake.
John Juan
New member
Thymosin beta4 and cardiac repair.
Shrivastava S, et al. Show all Journal
Ann N Y Acad Sci. 2010 Apr;1194:87-96. doi: 10.1111/j.1749-6632.2010.05468.x.
Affiliation
Abstract
Hypoxic heart disease is a predominant cause of disability and death worldwide. As adult mammals are incapable of cardiac repair after infarction, the discovery of effective methods to achieve myocardial and vascular regeneration is crucial. Efforts to use stem cells to repopulate damaged tissue are currently limited by technical considerations and restricted cell potential. We discovered that the small, secreted peptide thymosin beta4 (Tbeta4) could be sufficiently used to inhibit myocardial cell death, stimulate vessel growth, and activate endogenous cardiac progenitors by reminding the adult heart on its embryonic program in vivo. The initiation of epicardial thickening accompanied by increase of myocardial and epicardial progenitors with or without infarction indicate that the reactivation process is independent of injury. Our results demonstrate Tbeta4 to be the first known molecule able to initiate simultaneous myocardial and vascular regeneration after systemic administration in vivo. Given our findings, the utility of Tbeta4 to heal cardiac injury may hold promise and warrant further investigation.
Shrivastava S, et al. Show all Journal
Ann N Y Acad Sci. 2010 Apr;1194:87-96. doi: 10.1111/j.1749-6632.2010.05468.x.
Affiliation
Abstract
Hypoxic heart disease is a predominant cause of disability and death worldwide. As adult mammals are incapable of cardiac repair after infarction, the discovery of effective methods to achieve myocardial and vascular regeneration is crucial. Efforts to use stem cells to repopulate damaged tissue are currently limited by technical considerations and restricted cell potential. We discovered that the small, secreted peptide thymosin beta4 (Tbeta4) could be sufficiently used to inhibit myocardial cell death, stimulate vessel growth, and activate endogenous cardiac progenitors by reminding the adult heart on its embryonic program in vivo. The initiation of epicardial thickening accompanied by increase of myocardial and epicardial progenitors with or without infarction indicate that the reactivation process is independent of injury. Our results demonstrate Tbeta4 to be the first known molecule able to initiate simultaneous myocardial and vascular regeneration after systemic administration in vivo. Given our findings, the utility of Tbeta4 to heal cardiac injury may hold promise and warrant further investigation.
John Juan
New member
One of Thymosin Beta 4's key mechanisms of action is its ability to regulate the cell-building protein, Actin, a vital component of cell structure and movement. Of the thousands of proteins present in cells, actin represents up to 10% of the total proteins which therefore plays a major role in the genetic makeup of the cell.
captainslentz
New member
Any particular place known for quality tb500?
John Juan
New member
We carry USA made TB-500. Click on my signature banner, then at the site it's in alphabetical order in the research peptides section. There's a 30% discount code in my signature as well.
John Juan
New member
Thymosin Beta 4 was identified as a gene that was up-regulated four-to-six fold during early blood vessel formation and found to promote the growth of new blood cells from the existing vessels. This peptide is present in wound fluid and when administered subcutaneously, it promotes wound healing, muscle building and speeds up recovery time of muscles fibres and their cells.
