Thymosin Beta 4(TB-500) Injury Healing

[John Juan]

This potent peptide is a member of a ubiquitous family of 16 related molecules with a high conservation of sequence and localization in most tissues and circulating cells in the body. Thymosin Beta 4 not only binds to actin, but also blocks actin polymerization and is the actin-sequestering molecule in eukaryotic cells.

 

[John Juan]

Research confirms that T4 is a potent, naturally occurring wound repair factor with anti-inflammatory properties. T_¨_4 is different from other repair factors, such as growth factors, in that it promotes endothelial and keratinocyte migration. It also does not bind to the extracellular matrix and has a very low molecular weight meaning it can travel relatively long distances through tissues.

 

[John Juan]

TB-500 reduces stomach acid which may help eliminate ulcers.

 

[John Juan]

Animal studies with thymosin beta, a multifunctional tissue repair and regeneration peptide.

AuthorsPhilp D, et al. Show all Journal
Ann N Y Acad Sci. 2010 Apr;1194:81-6. doi: 10.1111/j.1749-6632.2010.05479.x.

Affiliation
Abstract
Studies in various animal models of disease and repair with thymosin beta(4) (Tbeta(4)), the major actin-sequestering molecule in mammalian cells, have provided the scientific foundation for the ongoing dermal, corneal, and cardiac wound repair multicenter clinical trials. Tbeta(4) has of multiple biological activities, which include down-regulation of inflammatory chemokines and cytokines, and promotion of cell migration, blood vessel formation, cell survival, and stem cell maturation. All of these activities contribute to the multiple wound healing properties that have been observed in animal studies. This paper reviews and discusses the topical and systemic uses of Tbeta(4) in various animal models that demonstrate its potential for clinical use.

PMID 20536453 [PubMed - indexed for MEDLINE]

 

[John Juan]

Thymosin beta4 increases hair growth by activation of hair follicle stem cells.

AuthorsPhilp D, et al. Show all Journal
FASEB J. 2004 Feb;18(2):385-7. Epub 2003 Dec 4.

Affiliation
Abstract
Thymosin beta4, a 43-amino acid polypeptide that is an important mediator of cell migration and differentiation, also promotes angiogenesis and wound healing. Here, we report that thymosin beta4 stimulates hair growth in normal rats and mice. A specific subset of hair follicular keratinocytes in mouse skin expresses thymosin beta4 in a highly coordinated manner during the hair growth cycle. These keratinocytes originate in the hair follicle bulge region, a niche for skin stem cells. Rat vibrissa follicle clonogenic keratinocytes, closely related, if not identical, to the bulge-residing stem cells, were isolated and their migration and differentiation increased in the presence of nanomolar concentrations of thymosin beta4. Expression and secretion of the extracellular matrix-degrading enzyme matrix metalloproteinase-2 were increased by thymosin beta4. Thus, thymosin beta4 accelerates hair growth, in part, due to its effect on critical events in the active phase of the hair follicle cycle, including promoting the migration of stem cells and their immediate progeny to the base of the follicle, differentiation, and extracellular matrix remodeling.

PMID 14657002 [PubMed - indexed for MEDLINE]

 

[John Juan]

I read an interview of a race horse doctor. He said the two most incredible horse racing drugs for racings performance were TB-500 and igf1-Lr3.

 

[John Juan]

Thymosin beta 4 peptide can cause neuronal regeneration.

 

[John Juan]

They're busting race horses for having an unfair advantage now. Hahaha does that mean they have to sit out a certain number of races like in baseball? Hahahaha

Doping control analysis of TB-500, a synthetic version of an active region of thymosin β₄, in equine urine and plasma by liquid chromatography-mass spectrometry.

AuthorsHo EN, et al. Show all Journal
J Chromatogr A. 2012 Nov 23;1265:57-69. doi: 10.1016/j.chroma.2012.09.043. Epub 2012 Sep 23.

Affiliation
Abstract
A veterinary preparation known as TB-500 and containing a synthetic version of the naturally occurring peptide LKKTETQ has emerged. The peptide segment (17)LKKTETQ(23) is the active site within the protein thymosin β(4) responsible for actin binding, cell migration and wound healing. The key ingredient of TB-500 is the peptide LKKTETQ with artificial acetylation of the N-terminus. TB-500 is claimed to promote endothelial cell differentiation, angiogenesis in dermal tissues, keratinocyte migration, collagen deposition and decrease inflammation. In order to control the misuse of TB-500 in equine sports, a method to definitely identify its prior use in horses is required. This study describes a method for the simultaneous detection of N-acetylated LKKTETQ and its metabolites in equine urine and plasma samples. The possible metabolites of N-acetylated LKKTETQ were first identified from in vitro studies. The parent peptide and its metabolites were isolated from equine urine or plasma by solid-phase extraction using ion-exchange cartridges, and analysed by liquid chromatography-mass spectrometry (LC/MS). These analytes were identified according to their LC retention times and relative abundances of the major product ions. The peptide N-acetylated LKKTETQ could be detected and confirmed at 0.02 ng/mL in equine plasma and 0.01 ng/mL in equine urine. This method was successful in confirming the presence of N-acetylated LKKTETQ and its metabolites in equine urine and plasma collected from horses administered with a single dose of TB-500 (containing 10mg of N-acetylated LKKTETQ). To our knowledge, this is the first identification of TB-500 and its metabolites in post-administration samples from horses.

 

[John Juan]

We left the 30% off discount code active today because someone always complains they were in a different time zone and the code didn't work when it should have. Take advantage of this and try out our thymosin beta 4(TB-500). Click on my banner for the website.

 

[John Juan]

TB-500 takes 6 weeks to fully build in the system. Even one vial per week does wonders for injuries.