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Info on Interleukin-15
- Thread starter badazzwhitedude
- Start date
badazzwhitedude
New member
Big BX said:
Yes there was a small image of a horses head on the vial.
badazzwhitedude
New member
CycloneHWT said:
lol. That reminds me of the scene in Nutty Professer II.
FreakMonster
New member
Imagine a human abusing this drug.
"Kentucky man takes IL-15 and weighs in at a muscular 500lbs. The man said he just wanted to gain an extra 100lbs more but ended up crushing his heart and killing him instantly. "
"Kentucky man takes IL-15 and weighs in at a muscular 500lbs. The man said he just wanted to gain an extra 100lbs more but ended up crushing his heart and killing him instantly. "
badazzwhitedude
New member
I will do some more research on this and see what I can come up with when I have some more time.
Hey ultra: Here's some info onIL-15, and where to buy it.
IL-15
Interleukin-15 (IL-15), a 18kD protein (162 amino acids), is a cytokine that stimulates the proliferation of T- lymphocytes. IL-15 high affinity receptor is composed of an IL-15-a chain plus the IL-2 receptor-b and -g chains. Thus, it has been shown that IL-15 can replace IL-2 in IL-2-dependent adult T-cell leukemia (ATL) cell lines, because IL-15 and IL-2 share common receptor components (Barzegar et al., 1998). In mice IL-15 has been shown to enhance NK cell activity in cytotoxicity assays using spleens from normal and tumor-bearing animals, and may serve as a useful anti-cancer adjuvant by activating initially the NK cell arm of the immune network (Evans et al., 1997). Human cells isolated from patients receiving IL-2 therapy contain NK cells expressing intermediate-affinity IL-2 receptor b/g complexes. Because IL-15 can activate cells through the IL-2 b/g receptors it is suggested that IL-15 might potentially be useful as an immunotherapeutic agent. Because low concentrations of IL-15 stimulates cytotoxicity against tumor targets, IL-15 could be used as a potential cancer immunotherapeutic agent instead of IL-2, as it displays less toxic side effects (de Jong et al., 1998).
Barzegar, C., Meazza, R., Pereno, R., Pottin-Clemenceau, C., Scudeletti, M., Brouty-Boye, D., Doucet, C., Taoufik, Y., Ritz, J., Musselli, C., Mishal, Z., Jasmin, C., Indiveri, F., Ferrini, S., and Azzarone, B. (1998). IL-15 is produced by a subset of human melanomas, and is involved in the regulation of markers of melanoma progression through juxtacrine loops. Oncogene 16: 2503-2512.
de Jong, J. L., Farner, N. L., Javorsky, B. R., Lindstrom, M. J., Hank, J. A., and Sondel, P. M. (1998). Differential quantitative effects of interleukin (IL)-2 and IL-15 on cytotoxic activity and proliferation by lymphocytes from patients receiving in vivo IL-2 therapy. Clin. Cancer Res. 4: 1287-1296.
Evans, R., Fuller, J. A., Christianson, G., Krupke, D. M., and Troutt, A. B. (1997). IL-15 mediates anti-tumor effects after cyclophosphamide injection of tumor-bearing mice and enhances adoptive immunotherapy: the potential role of NK cell subpopulations. Cell. Immunol. 179: 66-73.
http://members.aol.com/researchd/cytokines/rdicyt1.htm
IL-15 is a cytokine of approximately 15 kDa originally discovered as a T cell stimulatory activity (70) produced by activated monocytes, epithelial cells, and fibroblasts. IL-15 shares many biologic properties with IL-2 and mediates its activity via a multi-subunit high affinity receptor comprised of a unique alpha chain and the beta and gamma chains of the IL-2R. The human IL-15 gene has been mapped to chromosome 4 (70), similarly to IL-2. However, IL-15 does not exhibit any sequence homology with IL-2. IL-15 is produced by a large variety of cells including T lymphocytes and monocytes. It stimulates T lymphocyte and NK cell proliferation, as well as CTL and LAK activity (53). It enhances B cell expansion and immunoglobulin production (71) (Figure 2). It is also a T lymphocyte chemoattractant. IL-15 may be responsible for the recruitment and activation of T lymphocytes in the synovium of patients with rheumatoid arthritis where its levels have been found to be elevated (72).
Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
--------------------------------------------------------------------------------
DATA SHEET: recombinant Human INTERLEUKIN-15
-Interleukin 15 (IL-15) is a potent T-cell growth factor whose biological activity resembles that of IL-2 and which appears to interact predominantly with the B subunit of the IL-2 receptor (IL-2RB). This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Whereas an antibody to the ALPHA subunit of the IL-2 receptor was able to block IL-2 but not IL-15 in inducing T-cell proliferation, antibodies to IL-2RB consistently inhibit IL-15 more effectively than they inhibit IL-2. Additionally, it has been shown that neutralizing antibodies to Il-2 failed to block IL-15 activity in stimulating T-cell proliferation and induction of LAK cells. These data indicate that IL-15 acts independently of IL-2 and suggest that these two cytokines can act synergistically in stimulating the cellular immune response. Human IL-15 is a 13kDa protein containing 114 amino acid residues.
Catalog#: RDI-215
Package Size: 10 micrograms $170.00 or $150.00/pack 2 or more any
1 X 500ug=$2600.00 1 X 1mg=$4300.00
Mini-Pack: cat#RDI-215P $75.00/2ug
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis Endotoxin less than 0.1ng per ug of IL-15.
Reconstitution: Lyophilized IL-15 should be reconstituted in water or other aqueous buffer to a maximum concentration of 100 ng/ul. Allow to set at least 30 minutes at 4 DEG C , tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions or stored at -20 DEG C for future use. For most in-vitro applications, IL-15 exerts its biological activity in the range of 0.1 to 10.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.
Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted IL-15 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The specific activity is greater than 2 X 106 units per milligram as determined by the dose dependent stimulation of CTLL-2 cell proliferation (Ed50=<0.5ng/ml, corresponding to a specific activity of >2 million units per milligram.
AA Sequence:
MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA
MKCFLLELQV ISHESGDTDI HDTVENLIIL ANNILSSNGN
ITESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
--------------------------------------------------------------------------------
Return (RDI HOME PAGE)
Return (human cytokine index)
Research Diagnostics Inc
Pleasant Hill Road
Flanders NJ 07836
phone (973) 584-7093
fax (973) 584-0210
all rights reserved by any/all copyright holders?
IL-15
Interleukin-15 (IL-15), a 18kD protein (162 amino acids), is a cytokine that stimulates the proliferation of T- lymphocytes. IL-15 high affinity receptor is composed of an IL-15-a chain plus the IL-2 receptor-b and -g chains. Thus, it has been shown that IL-15 can replace IL-2 in IL-2-dependent adult T-cell leukemia (ATL) cell lines, because IL-15 and IL-2 share common receptor components (Barzegar et al., 1998). In mice IL-15 has been shown to enhance NK cell activity in cytotoxicity assays using spleens from normal and tumor-bearing animals, and may serve as a useful anti-cancer adjuvant by activating initially the NK cell arm of the immune network (Evans et al., 1997). Human cells isolated from patients receiving IL-2 therapy contain NK cells expressing intermediate-affinity IL-2 receptor b/g complexes. Because IL-15 can activate cells through the IL-2 b/g receptors it is suggested that IL-15 might potentially be useful as an immunotherapeutic agent. Because low concentrations of IL-15 stimulates cytotoxicity against tumor targets, IL-15 could be used as a potential cancer immunotherapeutic agent instead of IL-2, as it displays less toxic side effects (de Jong et al., 1998).
Barzegar, C., Meazza, R., Pereno, R., Pottin-Clemenceau, C., Scudeletti, M., Brouty-Boye, D., Doucet, C., Taoufik, Y., Ritz, J., Musselli, C., Mishal, Z., Jasmin, C., Indiveri, F., Ferrini, S., and Azzarone, B. (1998). IL-15 is produced by a subset of human melanomas, and is involved in the regulation of markers of melanoma progression through juxtacrine loops. Oncogene 16: 2503-2512.
de Jong, J. L., Farner, N. L., Javorsky, B. R., Lindstrom, M. J., Hank, J. A., and Sondel, P. M. (1998). Differential quantitative effects of interleukin (IL)-2 and IL-15 on cytotoxic activity and proliferation by lymphocytes from patients receiving in vivo IL-2 therapy. Clin. Cancer Res. 4: 1287-1296.
Evans, R., Fuller, J. A., Christianson, G., Krupke, D. M., and Troutt, A. B. (1997). IL-15 mediates anti-tumor effects after cyclophosphamide injection of tumor-bearing mice and enhances adoptive immunotherapy: the potential role of NK cell subpopulations. Cell. Immunol. 179: 66-73.
http://members.aol.com/researchd/cytokines/rdicyt1.htm
IL-15 is a cytokine of approximately 15 kDa originally discovered as a T cell stimulatory activity (70) produced by activated monocytes, epithelial cells, and fibroblasts. IL-15 shares many biologic properties with IL-2 and mediates its activity via a multi-subunit high affinity receptor comprised of a unique alpha chain and the beta and gamma chains of the IL-2R. The human IL-15 gene has been mapped to chromosome 4 (70), similarly to IL-2. However, IL-15 does not exhibit any sequence homology with IL-2. IL-15 is produced by a large variety of cells including T lymphocytes and monocytes. It stimulates T lymphocyte and NK cell proliferation, as well as CTL and LAK activity (53). It enhances B cell expansion and immunoglobulin production (71) (Figure 2). It is also a T lymphocyte chemoattractant. IL-15 may be responsible for the recruitment and activation of T lymphocytes in the synovium of patients with rheumatoid arthritis where its levels have been found to be elevated (72).
Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
--------------------------------------------------------------------------------
DATA SHEET: recombinant Human INTERLEUKIN-15
-Interleukin 15 (IL-15) is a potent T-cell growth factor whose biological activity resembles that of IL-2 and which appears to interact predominantly with the B subunit of the IL-2 receptor (IL-2RB). This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Whereas an antibody to the ALPHA subunit of the IL-2 receptor was able to block IL-2 but not IL-15 in inducing T-cell proliferation, antibodies to IL-2RB consistently inhibit IL-15 more effectively than they inhibit IL-2. Additionally, it has been shown that neutralizing antibodies to Il-2 failed to block IL-15 activity in stimulating T-cell proliferation and induction of LAK cells. These data indicate that IL-15 acts independently of IL-2 and suggest that these two cytokines can act synergistically in stimulating the cellular immune response. Human IL-15 is a 13kDa protein containing 114 amino acid residues.
Catalog#: RDI-215
Package Size: 10 micrograms $170.00 or $150.00/pack 2 or more any
1 X 500ug=$2600.00 1 X 1mg=$4300.00
Mini-Pack: cat#RDI-215P $75.00/2ug
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis Endotoxin less than 0.1ng per ug of IL-15.
Reconstitution: Lyophilized IL-15 should be reconstituted in water or other aqueous buffer to a maximum concentration of 100 ng/ul. Allow to set at least 30 minutes at 4 DEG C , tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions or stored at -20 DEG C for future use. For most in-vitro applications, IL-15 exerts its biological activity in the range of 0.1 to 10.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.
Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted IL-15 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The specific activity is greater than 2 X 106 units per milligram as determined by the dose dependent stimulation of CTLL-2 cell proliferation (Ed50=<0.5ng/ml, corresponding to a specific activity of >2 million units per milligram.
AA Sequence:
MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA
MKCFLLELQV ISHESGDTDI HDTVENLIIL ANNILSSNGN
ITESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
--------------------------------------------------------------------------------
Return (RDI HOME PAGE)
Return (human cytokine index)
Research Diagnostics Inc
Pleasant Hill Road
Flanders NJ 07836
phone (973) 584-7093
fax (973) 584-0210
all rights reserved by any/all copyright holders?
Your_Moms_Kneepads
New member
He'd never be able to do it, even if he could he wouldn't be able to afford the technology or raw materials. Hopefully in the future (like GH did) it is produced more,ect. and the price comes down.manny78 said:
The Ru-486 is something that I would suspect that he could manage to do. It would sell big.
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