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Whatman... .20 or .45 ?
- Thread starter anal itch
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!ns
New member
used 0.45 whatman for first test cyp batch, spent about 10minutes for filtering 50ml of solution. i think BA and baking do its job for sterilize solution, never had any issue with this test C
read that baking in the stove can destroy hormone and lead to lost gear potency, next time i will not do this
read that baking in the stove can destroy hormone and lead to lost gear potency, next time i will not do this
Wow so many mistakes.
.45 doesnt sterilize gear
Baking doesnt sterilize gear
BA doesnt sterilize gear.
Why would u not use a .2 filter?
That is all u need. Baking also removes the BA and now u r growing bacteria in an already non-sterile environment, very dangerous. I am not being insulting just trying to help you and anyone else reading this thread.
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Cant get much clearer than that, period! Well said!
phalaenopsis
New member
According to Pharmacopea for sterylisation .22 um filter is needed. It's beacuse only .22 um filter will remove all bacteria form solution. .45 filters are used to remove non-soluable compuunds - only for claryfication. Also very critical point is transfering sterile filtered solution to vials and crimping. You must have sterile vials, stoppers and flip-tops. Solution must be transfered from receiver bottle in sterile environment (clean room). But until we don't have clean rooms in our houses we must be very, very cearful. BA doesn't sterylise solution. It prevent growth of germs. Sterylisaton methods are:
1.dry heat sterylisation (160*C for 2h, 170*C for 1h, 180*C for 30 min - time is measured from point when material achieve diserable temp.)
2. autoclaving (121*C 0,1 MPa overpressure for 15min)
3. filtering (.22 um filters)
4. radiation (gamma from Cs137 or Co60 radionuclids)
5. chemical (ethylene oxide gas, glutaraldehyde etc)
So we can take from this list 1,3 and 5 metod.
First method for glass vials and glass rciever bottles. Take large clean bowl, fill with distilled water add 2,3 drop of detergent (something you use for washing dishes) and wash in it your vials, bottles etc. After that flush your bowl with water and fill with distilled water. Flush vials and other glass until it won't shave. Take clean and washed with 70% isoprohyl alcohol metal tray, put on it upsidedown your glass. Put it to the oven and bake in desired temp. for appropirate time. Bottles must stay in normal position for few minutes to evaporate water, after that wrap thread with aluminium foil and put to the oven.
When glass is baking you can use 5 method to sterylise stoppers and flip tops by washing in glutaraldehyde/baking soda/water solution 2/0,3/97,7 % for 3h at standrad temperature or in 70% isoprophyl alcohol for same time. Afters 3h flush it in sterile water. Fill clean glass pressure bottle with distilled water to half volume and close it. Put it on water bath for 2h. When rubber and flip tops are flushed and wet I put it in opened bowl to cooling oven to dry it. Rubbers are stored after drying in closed bowl which is opened only when i take rubber to put it on vial and crimp.
Filling vials in air is risky but all steps which we done and BA in gear should make our gear safe. I heard about people which don't wash vials and rubbers, don't filter gear only bake it and thet are alive and haven't any infection. Allright. Junkies even use many times one neddle to IV injections and they are alive. But we are not junkies. We live healthy lifestyle and develop our bodies and we don't want HCV or any other nasty diseases.
Sorry for my language. I'm foreign and still learning
1.dry heat sterylisation (160*C for 2h, 170*C for 1h, 180*C for 30 min - time is measured from point when material achieve diserable temp.)
2. autoclaving (121*C 0,1 MPa overpressure for 15min)
3. filtering (.22 um filters)
4. radiation (gamma from Cs137 or Co60 radionuclids)
5. chemical (ethylene oxide gas, glutaraldehyde etc)
So we can take from this list 1,3 and 5 metod.
First method for glass vials and glass rciever bottles. Take large clean bowl, fill with distilled water add 2,3 drop of detergent (something you use for washing dishes) and wash in it your vials, bottles etc. After that flush your bowl with water and fill with distilled water. Flush vials and other glass until it won't shave. Take clean and washed with 70% isoprohyl alcohol metal tray, put on it upsidedown your glass. Put it to the oven and bake in desired temp. for appropirate time. Bottles must stay in normal position for few minutes to evaporate water, after that wrap thread with aluminium foil and put to the oven.
When glass is baking you can use 5 method to sterylise stoppers and flip tops by washing in glutaraldehyde/baking soda/water solution 2/0,3/97,7 % for 3h at standrad temperature or in 70% isoprophyl alcohol for same time. Afters 3h flush it in sterile water. Fill clean glass pressure bottle with distilled water to half volume and close it. Put it on water bath for 2h. When rubber and flip tops are flushed and wet I put it in opened bowl to cooling oven to dry it. Rubbers are stored after drying in closed bowl which is opened only when i take rubber to put it on vial and crimp.
Filling vials in air is risky but all steps which we done and BA in gear should make our gear safe. I heard about people which don't wash vials and rubbers, don't filter gear only bake it and thet are alive and haven't any infection. Allright. Junkies even use many times one neddle to IV injections and they are alive. But we are not junkies. We live healthy lifestyle and develop our bodies and we don't want HCV or any other nasty diseases.
Sorry for my language. I'm foreign and still learning
Wow that sounds like a commercial job to me.
phalaenopsis
New member
In my country where salary is low as in Africa whole gym collect cash and make gear for themsleve.
Well you should all collect cash and get an autoclave and save time and money with all those extra steps. Good luck to you bro!
phalaenopsis
New member
It isn't so inconvinient as you think. In 8h with filtering you can make even 200 10ml vials of few types of gear. It's enough for few months. With autoclave you still have to wash and dry vials and filter with .45 filter to remove non-soluable compuunds. Also pharamcopea recommends for oil solutions dry heat sterylisation which will melt stoppers or may degradate testosterone (don't know for sure, never tried baked gear). Autoclaving will bring oil to 121*C but it won't make saturated vapor beacuse oil is free of water. In autoclaving saturated vapor takes key role in sterylisation by hydrolising and denaturating enzyms. Thats mean you will only heat in autoclave your oil to 121*C for 15 minutes when for dry heat sterylization you need 160*C for at least 2h.
I'm not crazy about extra clean conditions and safety beacuse I konw people are injecting unsterile crap in to their vains and nothing happen but I wan't to have psychical comfort and I'm aware we are far away from legit labs.
Good luck to you bro!
I'm not crazy about extra clean conditions and safety beacuse I konw people are injecting unsterile crap in to their vains and nothing happen but I wan't to have psychical comfort and I'm aware we are far away from legit labs.
Good luck to you bro!
I am sorry. You are misquoting me or making incorrect statements on others. In 8 hours I have seen 10L mixed, filtered and bottled. I am going to bed but will help you in the am. You really should look into chemiclaving. And gear has to be filtered at .22 first.
Good luck to you also my friend.
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