Thymosin Beta 4(TB-500) Injury Healing

[John Juan]

****CLICK ON MY BANNER BELOW FOR THE WEBSITE***



TB-500 helped my knees immensely. Just thought I would pass on what I learned. I dosed 2mg twice a week for one month. Prior to TB-500 I couldn't get off the toilet without using my hands to lift me off. This peptide does wonders for injury healing. Here is a recent study proving it speeds up healing of the MCL and other injuries. There are many studies regarding its healing properties.

Thymosin β4 enhances the healing of medial collateral ligament injury in rat.

AuthorsXu B, et al. Show all Journal
Regul Pept. 2013 Jun 10;184:1-5. doi: 10.1016/j.regpep.2013.03.026. Epub 2013 Mar 21.

ABSTRACT

The role played by thymosin β4 (Tβ4) in the process of wound healing was reported in several organs. However, there have been no reports that investigated the role of Tβ4 in the repair process after ligament injury. The purpose of this study was to determine whether administration of Tβ4 would improve ligament repair following injury. The medial collateral ligament (MCL) was sharply transected on the day of surgery. Then, the treatment group received 100 μL of fibrin sealant containing 1 μg of Tβ4 placed in the ligament gap. Healing tissues were evaluated by hematoxylin and eosin stain, transmission electron microscopy, and biomechanical test at 4 weeks after surgery. Histologically, healing tissues in Tβ4-treated group exhibited uniform and evenly spaced fiber bundles. However, the collagen fibers were not evenly spaced in control rats. Moreover, diameters of collagen fibrils within granulation tissue from the Tβ4-treated rats were significantly increased. In Tβ4-treated MCLs, the mechanical properties of these healing tissues were significantly higher at 4 weeks after surgery. In terms of the mechanical properties of the healing femur-medial collateral ligament-tibia complexes, the Tβ4-treated group had significantly better biomechanical properties than the control group at 4 weeks after surgery. Local administration of Tβ4 promotes the healing process of MCL, both histologically and mechanically, in a rat model. These findings provide a basis for potential clinical use of Tβ4 in repairing ligaments.

 

[Kevlor]

TB-500 is def one of the best peptides for injury recovery.

 

[John Juan]

Cardiac repair with thymosin β4 and cardiac reprogramming factors.

AuthorsSrivastava D, et al. Show all Journal
Ann N Y Acad Sci. 2012 Oct;1270:66-72. doi: 10.1111/j.1749-6632.2012.06696.x.

ABSTRACT
Heart disease is a leading cause of death in newborns and in adults. We previously reported that the G-actin-sequestering peptide thymosin β4 promotes myocardial survival in hypoxia and promotes neoangiogenesis, resulting in cardiac repair after injury. More recently, we showed that reprogramming of cardiac fibroblasts to cardiomyocyte-like cells in vivo after coronary artery ligation using three cardiac transcription factors (Gata4/Mef2c/Tbx5) offers an alternative approach to regenerate heart muscle. We have combined the delivery of thymosin β4 and the cardiac reprogramming factors to further enhance the degree of cardiac repair and improvement in cardiac function after myocardial infarction. These findings suggest that thymosin β4 and cardiac reprogramming technology may synergistically limit damage to the heart and promote cardiac regeneration through the stimulation of endogenous cells within the heart.

 

[John Juan]

Thymosin beta4 enhances repair by organizing connective tissue and preventing the appearance of myofibroblasts.

AuthorsEhrlich HP, et al. Show all Journal
Ann N Y Acad Sci. 2010 Apr;1194:118-24. doi: 10.1111/j.1749-6632.2010.05483.x.

Affiliation
Abstract
Incisional wounds in rats treated locally with thymosin beta4 (Tbeta4) healed with minimal scaring and without loss in wound breaking strength. Treated wounds were significantly narrower in width. Polarized light microscopy treated wounds had superior organized collagen fibers, displaying a red birefringence, which is consistent with mature connective tissue. Control incisions had randomly organized collagen fibers, displaying green birefringence that is consistent with immature connective tissue. Immunohistology treated wounds had few myofibroblasts and fibroblasts with alpha smooth muscle actin (SMA) stained stress fibers. Polyvinyl alcohol sponge implants placed in subcutaneous pockets received either carrier or 100 microg of Tbeta4 on days 2, 3, and 4. On day 14, treated implants revealed longer, thicker collagen fiber bundles with intense yellow-red birefringence by polarized light microscopy. In controls, fine, thin collagen fiber bundles were arranged in random arrays with predominantly green birefringence. Controls contained mostly myofibroblasts, while few myofibroblasts appeared in Tbeta4 treated implants. Electron microscopy confirmed both cell types and the degree of collagen fiber bundle organization. Our results demonstrate that Tbeta4 treated wounds appear to mature earlier and heal with minimal scaring.

 

[John Juan]

I had a minor pec tear tonight in my right pec while on a machine chest press. It looks like I'll be starting a log on TB-500 and BPC 157 ASAP. I've used TB-500 to heal my knees but this will be my first run with BPC 157. I'll administer the TB-500 subcutaneously and inject the BPC 157 intramuscularly as close to the point of injury as possible.

 

[John Juan]

Animal studies with thymosin beta, a multifunctional tissue repair and regeneration peptide.

AuthorsPhilp D, et al. Show all Journal
Ann N Y Acad Sci. 2010 Apr;1194:81-6. doi: 10.1111/j.1749-6632.2010.05479.x.

Affiliation
Abstract
Studies in various animal models of disease and repair with thymosin beta(4) (Tbeta(4)), the major actin-sequestering molecule in mammalian cells, have provided the scientific foundation for the ongoing dermal, corneal, and cardiac wound repair multicenter clinical trials. Tbeta(4) has of multiple biological activities, which include down-regulation of inflammatory chemokines and cytokines, and promotion of cell migration, blood vessel formation, cell survival, and stem cell maturation. All of these activities contribute to the multiple wound healing properties that have been observed in animal studies. This paper reviews and discusses the topical and systemic uses of Tbeta(4) in various animal models that demonstrate its potential for clinical use.

 

[John Juan]

A Superior customer who is using TB-500 posted that he feels a definite pain reduction in his knees when dosing TB-500 immediately prior to leg training. My guess is it is due to an antiinflammatory effect. He said it allows him to train much heavier and for longer periods of time on leg day.

 

[John Juan]

REDUCES INFLAMMATION....

Thymosin beta(4) reduces lethality and down-regulates inflammatory mediators in endotoxin-induced septic shock.

AuthorsBadamchian M, et al. Show all Journal
Int Immunopharmacol. 2003 Aug;3(8):1225-33.

Affiliation
Abstract
Thymosin beta(4) (Tbeta(4)), a highly conserved peptide with immunomodulatory properties, is the major actin-sequestering peptide in mammalian cells. Recent studies have established that Tbeta(4) can accelerate wound healing in full thickness skin wounds and following burn injuries to the cornea. In the eye studies, the accelerated healing due to Tbeta(4) was accompanied by a significant reduction in polymorphonuclear leukocyte (PMN) infiltration and a several-fold decrease in interleukin-1beta (p< or =0.015) and 6-keto-prostaglandin F(1alpha) (6-keto-PGF1alpha, p< or =0.05). Given the recognized role of proinflammatory cytokines in septic shock and of extracellular F- and G-actin in the pathophysiology of multiple organ dysfunction, we have investigated the role of Tbeta(4) in sepsis. We report that an LD(50) dose of LPS (24 mg/kg) in rats resulted in a significant reduction of Tbeta(4) levels in the blood. Furthermore, administration of 100 microg of Tbeta(4) immediately following and at 2 and 4 h after an LD(50) dose of LPS (60 mg/kg) in mice significantly reduced mortality rates (p< or =0.024) and lowered blood levels of a number of inflammatory cytokines, eicosanoids, and other molecules that are highly elevated following endotoxin administration. In studies in human subjects given low doses of endotoxin (4 ng/kg LPS) and in patients with septic shock, we have also observed significant decreases in blood levels of Tbeta(4). The rapid disappearance of Tbeta(4) in the blood following LPS administration or during septic shock suggests that Tbeta(4) may be involved in early events leading to activation of the inflammatory cascade and ultimately the clinical sequelae of sepsis. The results of this study indicate that Tbeta(4) may have utility in the clinic in the treatment of septic shock and in syndromes associated with actin toxicities.

 

[John Juan]

It's inflammation that causes much of the pain in the gym when we train. Swelling of tissues press up against nerves and it hurts. TB-500 reduces inflammation, as the study above proves, so besides the actual healing benefits you also get reduced pain in the gym.

 

[John Juan]

Thymosin is a hormone secreted from the thymus. Its primary function is to stimulate the production of T cells, which are an important part of the immune system