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From Media to Vial?
- Thread starter melochai
- Start date
phalaenopsis
New member
Check my pos in thread
Whatman... .20 or .45 ?
Whatman... .20 or .45 ?
melochai
New member
Maybe I should share what I have been doing and see what you all think. Maybe you have a better way, if so Let me know.
So, I start off sterilizing my bottle and caps, like I have read in the forums. I have 2 caps,one is a solid cap and one has a hole in it for a silicone septa. Once I get product ready for filtering and the media bottle is cool enough to handle with my bare hand, I attach filter and get to filtering. After filtering is done, I swab the silicone septa with an alcohol swab, much like you would the top of the vial prior to an injection. After the septa has dried I get it started in the cap, remove the filter from the bottle and install the septa cap. Then I basically just transfer it from the media bottle to the vials that are bought as sealed and sterile.
What do you think about it ?
So, I start off sterilizing my bottle and caps, like I have read in the forums. I have 2 caps,one is a solid cap and one has a hole in it for a silicone septa. Once I get product ready for filtering and the media bottle is cool enough to handle with my bare hand, I attach filter and get to filtering. After filtering is done, I swab the silicone septa with an alcohol swab, much like you would the top of the vial prior to an injection. After the septa has dried I get it started in the cap, remove the filter from the bottle and install the septa cap. Then I basically just transfer it from the media bottle to the vials that are bought as sealed and sterile.
What do you think about it ?
phalaenopsis
New member
It's OK. You can put septa in 70% isoprophyl alcohol for 3h and dry it in cooling oven wrapped in aluminium foil or in bowl with cover and use dryied when you need.
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