Best way to sterilize gear: bake, nuke or filter?

[ript1]

Hey guys, I need some advice bad, from anyone who's knowledgeable/serious, please!! I have a bunch of gear I transported in contact lens solution bottles. I want to use it now, and I have a few important questions:
1. Even though the bottles were supposedly sterile (brand-new contact lens solution bottles) and I transferred the gear into them using sterile syringes, I'm still concerned that during the transportation/storage process (stored for about 6 months after transportation) the gear might have gotten contaminated. First question: Do you think the gear needs to be sterilized before I use it?

2. What is the best way to sterilize gear? (I have oil-based and water-based)? I have read a bunch of postings and so far haven't found anything comparing the three methods that everybody has been talking about: baking, nuking and filtering.

I really need some help and I appreciate any advice, even links to the information I'm looking for. Thanks bros gotta start stackin for summer...PEACE

 

[doubles1]

bump good quest

 

[zarathustra]

I'm surprised you couldn't find anything on sterilizing gear, as I've seen many posts on this.

I've never had to do it, but I'm pretty sure using the microwave is a bad idea, mainly because microwaves can vary a great deal in power. The "high" setting on one persons microwave could be using much more, or much less power than your own (and thus heat the solution far more, or far less than expected).

I believe its common for oil based gear to be heated (baked) for 15 minutes at 212F, but that may not apply to all oil based injectables and their respective esters.

I'm sure there is some good info on this somewhere, you might just have to look a bit harder. At least you've already got the gear...some of us are having a hard time getting that far

 

[Seth28]

best sterilization method is filter with a .2micron and add a little BA to the gear. I researched the net looking for the answer and NOWHERE could i find that baking on 250 for 3 fifteen minute intervals would sterilize shit. I think the lowest temps i found was like 350 for 3 hours or something. I know some swear by it, but i couldnt find anything.

 

[Jman88]

This is from Animal's website:

If you are ordering oils such as fina and eq that are either homemade, IP
made, or some other underground manufacturer you should follow steps to ensure
a clean sterile injection. Below is the following steps I have outlined in
order to sterilize your product for a safe injection.

note: this also evaporates some of the BA so that the shot will be less
painful. I acheive a totally painless injection with fina this way.

Microwave Sterilization
This is the easiest method to use and fastest.

step 1 - YOU MUST TAKE THE METAL SEAL OFF THE TOP OF THE VIAL!

step 2 - Nothing should be covering the top of the vial. Take the seal and the
stopper off the top of the vial to where the oil is totally exposed. You can
do this with a pair of pliers or some instrument that can pry the lid off. The
seal will pry off pretty easily. I use a tool that is on my pocket knife. It
resembles a can opener.

step 3 - Place the vial in the microwave. Make sure there is nothing covering
the opening of the vial. Put the microwave on 20 seconds and hit start. After
it is through let sit for about 5 minutes and then repeat 2 more times.

step 4 - After the 3rd time immediately take the vial out of the microwave.
You may need mittens because it WILL be hot. At this point you should preload
all your syringes while the liquid is still hot. This is my preferred method
because it keeps everything sterile. Make sure to use 2 needles per syringe. 1
should be used to draw up the fluid. The other should be a clean one that will
be injected in your ass. The top of the second one should NOT BE REMOVED until
the time of injection. This ensures total sterility. If you do not want to
preload your syringes then youw ill need to put the rubber stopper back on the
syringe. Before you do that though plop it in the microwave for about 20
seconds on a paper towl or plate. Immediately take the stopper out and put it
on the vial using the napkin to pick it up with. This is a pain in the ass
though when the time comes to draw up the fluid. Not only do you expose the
oil once again when you take the seal off but you risk spilling the oil all
over the place. It is just easier to preload all the syringes when the oil is
still hot and sterile. Then you dont have to mess with it at a later date and
you can just inject

NOTE: I will be developing oil products soon. I will be using these same
techniques to sterilize the oils I will be selling. The vials will be
professionally sealed and will be ready to withdraw the fluid without first
sterilizing.

Boil
This takes some time cause you have to bring the water to a boil and then put
the vial in there. If you do not want to expose the oil because you will not
be preloading then I would use this method or the bake method.

step 1 - Stick a needle from a syringe through the rubber top of the vial so
that it is sticking through it by about a half an inch. The purpose of this is
to allow the BA to evaporate out of the vial. If it does not then the vial
WILL EXPLODE.

step 2 - Place the vial in a small pot. Fill the water level up to about a
half an inch below the top of the vial. Turn on the stovetop to about medium
high. You can even do high. (I do) Wait till the water starts boiling before
you start counting down the minutes. Let the vial sit in the water for 15
minutes then remove. (the vial may move around in the water as the bubbles
will push it around but it will not fall over)

step 3 - Sit the vial on a paper towl or other surface that can soak of any
water. Take the needle out of the top of the vial before it cools. If you let
it cool the needle will most likely get stuck there. It is actually smart to
leave it in there though. This way you can just put the syringe on needle and
draw up the fluid as you go. But if you do this make sure to put another
syringe on there when you are through with filling the other one. If you dont
you are leaving a small hole open to let bacteria get in. That is it for the
boil method. You can preload the syringes if you like after you take the vial
out. Beware though. It is a pain in the ass to attempt to preload the syringes
with the top on there and the liquid is still hot cause you will have to turn
it upside down to get the last bit of liquid in the syringes. That vial is
very hot!

Bake Method
Bakind is pretty much the same procedure as the boil method. Just place the
vial in a pan and put a needle in the top. Bake 15 minutes on 250 degrees.
Wait about 10-15 min and repeat 2 more times.


I hope this helps some of you guys that may be weary about ordering IP gear
and other vet products. All oils CAN be heat sterilized. If you take the
correct procedures you will have IMO human grade products after you heat
sterilize the product. Besides....test prop is test prop after all. It doesnt
matter if a pharmaceutical company produces it or IP. As long as the dosages
are correct it works the same way in your body. The only difference is human
grade is sterile shipped to you and IP is not always sterile. It is just smart
to heat sterilize it. For the money you cannot beat private lab made gear, and
if proper sterilization processes are use it is the same thing as human grade.


IMPORTANT!
If your oil is shipped to you in a plastic container of some sort you cannot
obviously put this in boiling water or the oven. You can however put this in
the microwave for 20 seconds. If it is in a plastic container you must let the
product totally cool though before reheating it 2 more times in the microwave
or you risk the plastic vial melting.

 

[JordanK25]

Great info man thanks.

 

[Peyomp]

I'm going with the filter and then heat gear in boiling water method.

 

[skeeber]

best sterilization method is filter with a .2micron and add a little BA to the gear.

I'm gonna go with Seth on this one. Check out this thread:

http://www.cuttingedgemuscle.com/Forum/showthread.php?s=&threadid=3796

 

[ript1]

Thanks you guys, all great posts. Although you seem to be a bit divided... As far as I can tell the general consensus is that filtering with a .2 micron filter will be effective in removing most harmful bacteria and that heating (with temperature alternation) may also kill some bacteria, but perhaps is not as effective as filtering.

The info on the heating method (Jman88, above) is awesome, but I still don't know how to use a filter. Since I have some water-based gear I'll need to go through that as well. I saw I can order them from anabolicfitness.com though. Any info or links would be welcomed.

Also, what is BA and where can I get it?

Again, I really appreciate the help. I don't have anyone else I can ask about this.

 

[ript1]

Gotta bump myself, shamelessly.

Anybody out there tell me how much liquid I can filter through one filter, how to use the filter and what the hell is BA??????

Again, thanks for all the advice, and further help or links are truly appreciated.
Peace men.