Whatman... .20 or .45 ?

[phalaenopsis]

I must misunderstand you. So when you wrote about autoclaving you had on your mind autoclaving only vials and rubberstops not final bottled product? Maybe you know degradating temperatures for different types of hormnes?
Regards

 

[goin on 4T]

I must misunderstand you. So when you wrote about autoclaving you had on your mind autoclaving only vials and rubberstops not final bottled product? Maybe you know degradating temperatures for different types of hormnes?
Regards

You will not degrade hormone by autoclaving after vials are capped. It's well below degradation temp.


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[Tux]

So after I make my gear, I can autoclave it at a hospital to sterilize it without worry? Totally awesome dude!

 

[goin on 4T]

hospital?
or tatoo shop...
Or get your own autoclave.

 

[snutten_00]

Wondering one thing only, Shoud i use sterile or nonsterile filters when I must filter finished product ?

 

[AnthonyMarco]

Wondering one thing only, Shoud i use sterile or nonsterile filters when I must filter finished product ?

When in doubt, sterile.

Non-sterile is awesome as long as you have a way to sterilize them since they are so much cheaper, but what happens is non-sterile have bacteria on/in the exit port which goes right in your vial.

In practice, the benzyl alcohol will kill any bacteria getting past filtration, but why risk it. There are some bacteria you might not survive.

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[goin on 4T]

When in doubt, sterile.

Non-sterile is awesome as long as you have a way to sterilize them since they are so much cheaper, but what happens is non-sterile have bacteria on/in the exit port which goes right in your vial.

In practice, the benzyl alcohol will kill any bacteria getting past filtration, but why risk it. There are some bacteria you might not survive.

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BA does not kill bacteria. It is there to keep it from growing.

 

[AnonCanada]

Honestly, i moved from Whatman .20 and .45 filters over to a Nalgene Vacuum filter at .25 microns and am MUCH happier with the result. Cheaper over the long run an paying 3 dollars per whatman filter as well. And the Nalgene is much quicker.

That being said, if you only have access to the syringe filters, the .45 microns are fine.

 

[goin on 4T]

Honestly, i moved from Whatman .20 and .45 filters over to a Nalgene Vacuum filter at .25 microns and am MUCH happier with the result. Cheaper over the long run an paying 3 dollars per whatman filter as well. And the Nalgene is much quicker.

That being said, if you only have access to the syringe filters, the .45 microns are fine.

No its not fine to use a .45. Do you have any idea the size of bacteria? Do you how much can pass through as small as .3?

This is horrible information please stop telling people this as it could cause and abscess.

 

[AnthonyMarco]

BA does not kill bacteria. It is there to keep it from growing.

Really? I have always been under the impression it killed it.
I have used .45 before with no problems whatsoever, but based on this it looks like I was just lucky.

I retract my previous post. Only use .2 or .22 micrometer pore size filters for filtering your injectables. It is really the ONLY way.

Don't risk your health/life to save a couple dollars or a couple days.