aky

In 1997 McPherron and Lee from John Hopkins University discovered a gene that could be responsible for abnormal muscular growth in cattle. It is called the Myostatin gene (nickname " Schwarzenegger Gene " ). The gene produces a protein called Myostatin that regulates muscular growth. Experiments where the genes were mutated in Belgian Blue and Piedmontse cattle led to increased muscularity. Gains of up to 30% above normal levels of muscularity have been shown in cattle that experienced Myostatin mutations.

Shortly after the discovery of the Myostatin gene in cattle the gene was discovered in
mice. Mutation of the gene in mice resulted in 200-300% increases in muscle mass. The studies showed us that Myostatin had the same biological function in cattle and mice. These discoveries led to further investigations of the Myostatin gene. Scientists have discovered the gene in other vertebra animals including pigs, chickens, turkeys and humans.

Recent reports state that KFC is working on producing larger chickens through Myostatin mutation. Sources also say that McDonald's is working on a supercow
that is six times larger than normal cows. These reports are purely speculation; they are not official at this time.

Myostatin belongs to a family of molecules called transforming growth factors beta
(TGF-b ). It is also called ( GDF-8 ) growth and differentiation factor - 8. TGFb
subtypes are based on their related structures. GDF is one of these structures which
specifically regulates growth and differentiation. In the beginning researchers thought
Myostatin was present only in skeletal muscle. Since then, a New Zealand team of
researchers detected Myostatin in cardiac muscle. Research at Purdue University
detected the Myostatin gene in lactating mammary glands of pigs.

Myostatin plays a role in prenatal muscular growth. At this time we have little knowledge of Myostatins role in muscle regeneration. The process of muscle
regeneration is a complex situation. In young animals hormones and growth factors are likely to induce growth. As animals become adults these factors become down regulated. The muscles ability to express protein synthesis is lessened.

MyoD, IGF-I and myogenin are factors in determining a muscle cells specific characteristics. Muscle regulatory factor 4 ( MRF-4 ) mRNA expression is the dominant factor in adult muscular growth. As you can see in addition to Myostatin there are other factors involved in the regulation of muscle.

At this time little research has been performed on human models. Although the research that has been done shows that Myostatin works basically the same in humans as it does in animals.

Studies indicate Myostatin may control muscle fiber type.

Research also shows that altering the bodies metabolism has no effect on Myostatin
expression. Studies done on piglets and mice show that food restriction as well as
administration of exogenous growth hormone did not effect Myostatin levels. These
studies suggest that Myostatin expression is largely associated with prenatal muscular
growth.

High levels of Myostatin have been detected in HIV-infected men in comparison to
healthy males. This does not necessarily mean that Myostatin is a factor in muscle
wasting. At the present time we can not be sure of Myostatin's role in muscle regulation. Several authors suggest that Myostatin plays a larger role in muscle
regeneration after injury. One study reported that mutations in the human Myostatin gene did not play a role in altering muscle mass in weight trained subjects.

How does Myostatin apply to bodybuilding? This topic has been mentioned by various authorities in the bodybuilding industry. Mauro Dipasquale stated in a
interview with Testosterone magazine that Myostatin could be the wave of the future.
Dan Duchaine and Bill Roberts also stated that Myostatin inhibition could take top
level physiques to an even higher level.

Further research needs to be done with humans before we fully understand Myostatins role in human muscle. Some of the research done on animals looks very
promising, but we are not animals. The biggest problem facing Myostatin research at
this time is funding for this type of research.

Federal funding is unlikely for performance enhancement research. Scientists believe it is possible that inhibition of Myostatin could possibly help in treating muscle wasting
diseases. If this theory proves reliable it is possible that federal authorities would support Myostatin research for medical purposes.

In conclusion, Myostatin presents great possibilities, but at this time we do not have
sufficient evidence to determine Myostatin's role in humans.

References
Canada's Weekly News Review ( 1999 ) [ online ] available : http:
//ourpress.com/kfc_superchicken.htm [ 1999,Sept.28 (volume 1 , issue 20)

Falcon , M ( no date ) An overview of possible pathways to increased
muscular size with Dr. Lester Lee . Available : http// www.maxmuscle.com/research/expanding_genes/default.htm

Volk , E ( 1999 ) The Myostatin Gene [ online ] Available :
http//www.thinkmuscle.com/exclusive/volk/myostatin.htm [ 1999 , December
13 (Volume 2 , Number 20 ) www.halesoptimumphysique.com
COPYRIGHT 2000 JAMIE HALE

Bare minimum of 2 years before anyone will be able to get their hands on this.

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"Further research needs to be done with humans before we fully understand Myostatins role in human muscle. Some of the research done on animals looks very
promising, but we are not animals. The biggest problem facing Myostatin research at
this time is funding for this type of research.

Federal funding is unlikely for performance enhancement research. Scientists believe it is possible that inhibition of Myostatin could possibly help in treating muscle wasting
diseases. If this theory proves reliable it is possible that federal authorities would support Myostatin research for medical purposes."

It is for these reasons that you will not be able to come accross this for a long time.

It may be possible that someone with necessary funding will carry out the research needed in the expectations of owning the patent for this drug. As it stands there is already one man carrying out research on this and how safe it is for human use.

yall wanna try it ?? here it is , AT 200$ US per .1mg. and 1000$ for a full 1mg.

Cat. No.: RD181005220
Introduction:
Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor-� superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.
Specification: Anti-Human Myostatin Antibody was raised in bovine against recombinant human myostatin.
Specificity: The recombinant human myostatin is 100% homologous with the human serum myostatin.
Source of Antigen: E. coli
Host: Bovine
Purification Method: Immunoaffinity chromatography on a column with immobilized human myostatin
Antibody Content: 0.1 mg (determined by BCA method)
Titer: ELISA: >1:100,000 for antibody concentration 1 mg/ml
Formulation: Sterile filtered and lyophilized from 2 mg/ml in 0.2 M phosphate buffer, 0.2 M NaCl, pH 7.2
Reconstitution: Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage: Store lyophilized antibody at 2-8�C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80�C. Reconstituted antibody can be stored at 4�C for a limited period of time; it does not show decline in activity after two weeks at 4�C.
Stability/Shelf Life: The lyophilized antibody remains stable and fully active until the expiry date when stored at 4�C.
Quality Control Test: ELISA - to determine titer of the antibody
Applications: ELISA, Western blotting
Note: The Anti-Human Myostatin Bovine Polyclonal Antibody is for research use only.

References:
McPherron A.C. and Lee S.J.: Double muscling in cattle due to mutations in the myostatin gene. Proc. Natl. Acad. Sci. USA 94, 12457-12461 (1997)

Sharma M. et al.: Myostatin, a transforming growth factor � superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct. Journal of Cellular Physiology 180:1-9 (1999)

Gonzalez-Cadavid N.F., Taylor W.E. et al.: Organization of the human myostatin gene and expression in heathy men and HIV-infected men with muscle wasting. Proc. Natl. Acad. Sci. USA 95, 14938-14943 (1998)

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[This message has been edited by BigWh1tey (edited December 27, 2000).]

I didn't read anything about andications or administration.

I think it's a scam though, BioVendor that is. They didn't take my CC# or ask for payment options. They just wanted an oder number which I assume is a purchase order number.

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----BeMassive----

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Check out my HardCore Pic sitehttp://www.dell.homestead.com/conan/index.html

Has anyone given thought to the possibility that perhaps AAS turn off the Myostatin gene? I'm also curious how they are detecting the presence of a muscle gene in the blood? Perhaps they are measuring spillover myostatin from muscle and the use of AAS is turning off protein expression in the muscle.

Anyone have a ref. on the work done with Flex and other bodybuilders?

Three type of blottings exist:
Southern Blot, which analyzes DNA
Northern Blot, which analyzes RNA
and Western Blot, which analyzes proteins.

Here's a description of western blotting:
(MORE COMMONLY REFERED TO AS Gel Electrophoresis)

Western blotting is just a technique to determine what type of proteins can exist in any sample (for example, an onion skin, human blood, hair, etc.) by separating the proteins based on molecular weight. An electric current is run through a gel, which pushes the proteins of various weights across the gel. Then from the "photograph" the proteins leave behind, you can tell what proteins are in the sample.

Here's a better website to describe the method:
http://esg-www.mit.edu:8001/esgbio/rdna/rdna.html

I am also going to talk to a professor to find out more about this particular protein. This weekend I am going to go to the library and look on Lexus nexus to look into any medical research that has been done with Myostatin. Just a start, should be interesting.

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----BeMassive----